Traumatic mind damage (TBI) outcomes from exterior mechanical forces inflicting mind dysfunction. Dysregulated microRNAs (miRNAs) have been implicated in TBI pathophysiology, however the function of miR-598-3p stays unclear. This research aimed to guage the diagnostic and prognostic worth of miR-598-3p in TBI and discover its regulatory results on irritation in an LPS-induced microglial damage mannequin. Serum miR-598-3p ranges have been measured by way of RT-qPCR in 137 TBI sufferers and 120 controls. ROC curves assessed its diagnostic and prognostic utility. TBI severity was categorised by Glasgow Coma Scale (GCS) scores, and outcomes have been evaluated utilizing the Glasgow Final result Scale (GOS). In vitro, LPS-treated BV2 microglia have been transfected with a miR-598-3p inhibitor to investigate inflammatory cytokine ranges. miR-598-3p expression was considerably elevated in TBI sufferers versus controls (p < 0.001) and elevated progressively with damage severity. ROC evaluation demonstrated excessive diagnostic accuracy for distinguishing TBI severity (AUC = 0.892/0.905) and predicting poor prognosis (AUC = 0.891, sensitivity = 83.33%, specificity = 90.36%). Excessive miR-598-3p expression correlated with elevated Marshall grades, neural damage markers (S100B, GFAP), and pro-inflammatory cytokines (IL-6, TNF-α). Multivariate evaluation recognized miR-598-3p as an impartial threat issue for poor outcomes (OR = 5.03, p < 0.01). miR-598-3p inhibition attenuated LPS-induced IL-6/TNF-α secretion (p < 0.01). Serum miR-598-3p demonstrates scientific utility as a biomarker for TBI analysis, severity grading, and prognostic analysis. Its noticed affiliation with amplified microglial inflammatory responses suggests a potential mechanism involving regulation of neuroinflammation pathways.
Key phrases:
diagnostic biomarker; miR‐598‐3p; neuroinflammation; prognostic biomarker; traumatic mind damage.
